| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Valerio, Sara A (7)
|
MRYMID |
40.40 |
40.57 |
0.17 |
| 2 |
Benerofe, Noa S (7)
|
MREAST |
41.56 |
42.55 |
0.99 |
| 3 |
Euyoque, Nicole (7)
|
MRYMID |
40.63 |
42.89 |
2.26 |
| 4 |
Babyak, Eliza G (7)
|
MREAST |
40.84 |
43.28 |
2.44 |
| 5 |
Costabile, Kylie (7)
|
MRCFB |
46.45 |
46.04 |
-0.41 |
| 6 |
Bartolacci, Abigail J (7)
|
MRRA |
48.10 |
46.65 |
-1.45 |
| 7 |
Nam, Lauren (7)
|
MRLIAC |
44.77 |
47.95 |
3.18 |
| 8 |
Cook, Keiran R (7)
|
MRLIAC |
49.09 |
54.15 |
5.06 |
| 9 |
Burriss, Leah S (7)
|
MREAST |
|
1:03.03 |
- |
| 10 |
Gregersen, Leah (6)
|
MRRA |
1:07.68 |
1:03.77 |
-3.91 |
| 11 |
McCann, Evelyn E (6)
|
MRYMID |
58.19 |
1:04.75 |
6.56 |
| 12 |
Benjamin, Mia (7)
|
MRRA |
1:18.03 |
1:18.79 |
0.76 |
|
Martinez-Jimenez, Alba (6)
|
MRYMID |
1:07.69 |
NS |
- |
|
Brennan, Jane E (7)
|
MRRA |
43.48 |
NS |
- |